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Comparison of three different extraction methods for Turbellaria
Martens, P.M. (1984). Comparison of three different extraction methods for Turbellaria. Mar. Ecol. Prog. Ser. 14: 229-234
In: Marine Ecology Progress Series. Inter-Research: Oldendorf/Luhe. ISSN 0171-8630; e-ISSN 1616-1599
Peer reviewed article  

Available in  Author 
    VLIZ: Open Repository 128972 [ OMA ]

Keywords
    Turbellaria [WoRMS]
    Marine

Author  Top 
  • Martens, P.M.

Abstract
    Three different extraction methods for Turbellaria were compared, 2 of which use living material. The first method is based on sea-water ice treatment (Uhlig-method), the second employs decantation of narcotized fauna (MgCl2-method). The third method is the Barnett-method, performed on preserved material. Three series of 12 samples each were analysed quantitatively. Turbellaria were counted and identified to the order level. For the turbellarian population as a whole, the numbers of individuals extracted alive (Uhlig- or MgCl2-method) was less than 50% of the number extracted from the preserved samples (Barnett-method). Differences between Uhlig-method and MgCl2-method were not statistically significant. However, for Acoela and Retronectida, the Uhlig-method was more efficient than both other methods. We conclude that for a reliable quantitative analysis of Turbellaria 2 different extraction methods are required: one should employ extraction of living material (Uhlig-method) and one extraction of fixed material. For qualitative investigations extraction of living material is recommended.

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