1. Species Gambusia affinis (Baird & Girard, 1853)
2. Species Gambusia dominicensis Regan, 1913
3. Species Gambusia heterochir Hubbs, 1957
4. Species Gambusia holbrooki Girard, 1859
5. Species Gambusia lemaitrei Fowler, 1950
6. Species Gambusia luma Rosen & Bailey, 1963
7. Species Gambusia manni Hubbs, 1927
8. Species Gambusia marshi Minckley & Craddock, 1962
9. Species Gambusia nicaraguensis Günther, 1866
10. Species Gambusia puncticulata Poey, 1854
11. Species Gambusia rhizophorae Rivas, 1969
12. Species Gambusia sexradiata Hubbs, 1936
13. Species Gambusia wrayi Regan, 1913
14. Species Gambusia xanthosoma Greenfield, 1983
15. Species Gambusia aestiputeus Fowler, 1950 accepted as Gambusia nicaraguensis Günther, 1866
16. Species Gambusia aestiputius Fowler, 1950 accepted as Gambusia nicaraguensis Günther, 1866 (misspelling)
17. Species Gambusia cascajalensis Meek & Hildebrand, 1913 accepted as Brachyrhaphis cascajalensis (Meek & Hildebrand, 1913)
18. Species Gambusia caymanensis Regan, 1913 accepted as Gambusia puncticulata Poey, 1854
19. Species Gambusia dovii Regan, 1913 accepted as Gambusia nicaraguensis Günther, 1866
20. Species Gambusia fasciata Meek, 1904 accepted as Poeciliopsis fasciata (Meek, 1904)
21. Species Gambusia gracilior Regan, 1913 accepted as Gambusia wrayi Regan, 1913
22. Species Gambusia gracilis Girard, 1859 accepted as Gambusia affinis (Baird & Girard, 1853)
23. Species Gambusia holbrocki Girard, 1859 accepted as Gambusia holbrooki Girard, 1859 (misspelling)
24. Species Gambusia holbrookii Girard, 1859 accepted as Gambusia holbrooki Girard, 1859 (misspelling)
25. Species Gambusia howelli Rivas, 1944 accepted as Gambusia puncticulata Poey, 1854
26. Species Gambusia hubbsi Breder, 1934 accepted as Gambusia puncticulata Poey, 1854
27. Species Gambusia humilis Günther, 1866 accepted as Gambusia affinis (Baird & Girard, 1853)
28. Species Gambusia mcneili Fowler, 1916 accepted as Gambusia nicaraguensis Günther, 1866 (misspelling)
29. Species Gambusia mcnieli Fowler, 1916 accepted as Gambusia nicaraguensis Günther, 1866
30. Species Gambusia melanosticta Regan, 1913 accepted as Gambusia puncticulata Poey, 1854
31. Species Gambusia modesta Troschel, 1865 accepted as Poecilia sphenops Valenciennes, 1846 (synonym)
32. Species Gambusia nigropunctata Regan, 1913 accepted as Gambusia puncticulata Poey, 1854
33. Species Gambusia oligosticta Regan, 1913 accepted as Gambusia puncticulata Poey, 1854
34. Species Gambusia patruelis (Baird & Girard, 1853) accepted as Gambusia affinis (Baird & Girard, 1853)
35. Species Gambusia picturada Poey, 1868 accepted as Gambusia puncticulata Poey, 1854
36. Species Gambusia plumbea Troschel, 1865 accepted as Poecilia sphenops Valenciennes, 1846
37. Species Gambusia turrubarensis Meek, 1912 accepted as Poeciliopsis turrubarensis (Meek, 1912)

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